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DMEM medium

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Thermofisher

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Lipofectamine™ 2000 Transfection Reagent
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Lipofectamine™ 3000 Transfection Reagent
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Penicillin-streptomycin (10,000 U/mL)
The solution contained 10,000 units/mL of penicillin and 10,000 μg/mL of streptomycin. Antibiotics – Penicillin and streptomycin have a potent combined antimicrobial effect against gram-positive and gram-negative bacteria and can be used to prevent bacterial contamination of cell cultures. Penicillin was originally purified from the fungus Penicillium and works by directly interfering with the synthesis of the bacterial cell wall and indirectly by triggering the release of enzymes to further alter the cell wall. Streptomycin was originally purified from Streptomyces gray. Its mechanism of action is binding to the 30S subunit of the bacterial ribosome, which in turn inhibits protein synthesis and leads to the death of sensitive bacteria.

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Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced Serum Medium is a modified minimum essential medium (MEM) capable of reducing fetal bovine serum addition by at least 50% with no change in growth rate or morphology. Opti-MEM™ I Medium is also recommended for use with cationic lipid transfection reagents such as Lipofectamine™ reagent. Opti-MEM™ I Medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Opti-MEM™ I modified media with a wide range of components are available for a wide range of cell culture applications.

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DMEM, high in sugar, with pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that can be used to support the growth of many species of mammalian cells. Cells that have been successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, and cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a wide range of DMEM modified media for a wide range of cell culture applications.

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UltraPure™ DNase/RNase-free distilled water
UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. Filtration through a 0.1-μm membrane, and DNase and RNase activity tests.

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Centrifolgaar Urtra Filation Tubes

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Brand pipettes

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Millex-GP Syringe Filter Unit, 0.22 µm, polyethersulfone, 33 mm, gamma sterilized

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Immobilon-P roll film, PVDF, 0.45 μm, 26.5 cm x 3.75 m

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Centrifugal ultrafiltration tubes

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